Job description

A research technician/contractor position is available at client

Must Have

• Biochemistry such as: Western blot, Immunofluorescence, flow cytometry
• Tissue culture exp is a must have
• Mammalian Cell culture exp is a must have
• Mammalian cell culture expertise: maintenance, freezing, thawing, and passaging.
• Molecular biology such as: Nucleic acid extraction, cDNA synthesis, RT-qPCR, ddPCR

We are looking for a highly-motivated research technician to work independently and in collaboration with other scientists on projects related to recombinant Adeno-Associated Virus (rAAV) production. Activities will be centered around the development of an inducible production system, quality control assays, and standard operating procedures.

• Perform stable HEK-293 cell line development processes including vector construction, transfection, cloning, cell line characterization, and cell banking
• Conduct small-scale AAV vector production and purification
• Characterize productivity and quality attributes
• Keep accurate records of experimental setup, SOP protocols, data quality control notes, etc.
• Maintain efficient and continuous communication with team members and managers

What hours and days will this person be working?

• Monday to Friday, with expectations to work on weekends and some holidays.

What are the top 5 skills/requirements this person is required have?

• Required Skill 1: Significant mammalian cell culture expertise: maintenance, freezing, thawing, and passaging.
• Required Skill 2: Biochemistry such as: Western blot, Immunofluorescence, flow cytometry
• Required Skill 3: Molecular biology such as: Nucleic acid extraction, cDNA synthesis, RT-qPCR, ddPCR

What years of experience, education, and/or certification are required?

• BS degree or master’s degree in biological sciences, molecular biology, neuroscience, or biochemistry and significant 3-5 years tissue culture experience are a must. (Or a master’s degree with at least 2 year of experience).
• The applicant should already be trained in proper wet lab procedures and should be familiar with using basic lab equipment. Having strong communication, organizational and documentation skills is a must.

What is a nice to have (but not required) regarding skills, requirements, experience, education, or certification?

• Previous experience working with AAV; molecular biology techniques such as plasmid construction, sequencing and QC assays; quantitative PCR, digital droplet PCR; RNA, DNA and protein extractions from cell or tissue samples; next generation sequencing (NGS) library preparation.

What is the environment that this person will be working in (i.e., group setting vs individual role)?

• The candidate will be involved in a highly enthusiastic, motivated, supportive, and diverse team. The candidate will be involved in multidisciplinary projects across different teams in GRC and is expected to work independently and support the team.

Is any testing required for this role? No

Does this position offer the ability to work remotely on a regular basis or is it an on-site role? If remote opportunity exists, what are the options (i.e., 1 day remote, 4 days on-site)? On site role

Other notable details about the environment from the hiring manager about this role

• Ability to participate in manuscripts preparation and authorship. Working schedule is flexible.

What positions/background experience do you feel are successful in this role?

• Research technicians, lab managers, research technologists, a master’s student with significant cell culture expertise

Job Type: Contract

Salary: $35.00 - $42.00 per hour

Schedule:

• 8 hour shift
• Monday to Friday

Ability to commute/relocate:

• North Chicago, IL 60064: Reliably commute or planning to relocate before starting work (Required)

Education:

• Bachelor's (Preferred)

Experience:

• Mammalian cell culture: 2 years (Preferred)
• Western blot, Immunofluorescence, flow cytometry: 3 years (Preferred)
• Nucleic acid extraction, cDNA synthesis: 3 years (Preferred)
• RT-qPCR, ddPCR: 3 years (Preferred)

Work Location: One location

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